Table 1 Summary of Lee County Mosquito Control District’s most significant lessons learned

Increased production rate with larval rearing system and pupal separation system

Optimized workflow with moving from pupal irradiation to adult irradiation

Reduced burden of data entry and analysis with the development of an in-house database that was curated specifically for our SIT program

References values from the beginning are essential for quality assurance

Product inconsistencies in larval diet and blood can lead to unexpected colony complications