Table 2 Steps involved with pupal and adult irradiation with associated times
From: Overview of the sterile insect technique for Aedes aegypti in Lee County, Florida, USA
Pupal irradiation | |
|---|---|
Task | Time duration |
Individual pupal weights | 30−40 min |
Return to holding container for overnight | Max. 16 h |
Drying and aliquoting | Max. 2 h |
Loading canisters, irradiation, removing pupae | 10 min, repeated 3 times |
Place in emergence containers with sugar | 30 min |
Emergence | ~ 36−48 h |
Draining | 2 h |
Dry overnight | Max. 16 h |
Marking | 1 h |
Ready for release | Total time: up to 86.67 h |
Adult irradiation | |
|---|---|
Task | Time duration |
Individual pupal weights | 30−40 min |
Drying and aliquoting | Max. 2 h |
Place in emergence containers with sugar | 30 min |
Emergence | ~ 36−48 h |
Draining | 2 h |
Dry containers | 30–60 min |
Cold knockdown | 75 min, repeated 2−3 times |
Packaging | 30 min, repeated 2−3 times |
Loading canisters, irradiation, removing adults | 5 min, repeated 2−3 times |
Marking and return to container | 15 min, repeated 2−3 times |
Ready for release | Total time: up to 60.42 h |